Supplementary MaterialsS1 Desk: Code for MBP and MBP+hBT shots

Supplementary MaterialsS1 Desk: Code for MBP and MBP+hBT shots. from mice injected with maltose binding proteins (MBP; control), MBP-human betatrophin (hBT) fusion proteins, or different concentrations of lipase inhibitor (raises serum triglyceride) and followed for just two times. Final bodyweight (g) and arbitrary fed blood sugar (mg/dl). Serum triglycerides (mg/dl) had been assessed at 0, 1, 3, 6, and a day after treatment.(XLSX) pone.0159276.s003.xlsx (47K) GUID:?26B0D35D-4A2C-4A7E-B594-BA4621994ECB S4 Desk: Person data for -cell proliferation evaluation from all three labs for ANGPTL8 treatment studies. (top panel) Total -cells counted for Ki67, total Ki67+ insulin+ DAPI+ cells, and Ki67+ -cells (% of total insulin+ cells) for the tail pancreas. (bottom panel) 10-DEBC HCl Total -cells counted, total EdU+ insulin+ DAPI+ cells, and EdU+ -cells (% of total insulin+ cells). 10-week-old CD1 male mice injected with buffer with or without EdU, or injected with MBP or MBP+hBT receiving EdU.(XLSX) pone.0159276.s004.xlsx (62K) GUID:?B74715BD-A66D-4BAA-8624-AABED59E0044 S5 Table: Individual data for Ki67, EdU co-expressing total cell and -cell analysis. Measurements were recorded from 10-week-old CD1 male mice injected with maltose binding protein (MBP; control) or MBP-human betatrophin (hBT) fusion protein for two days and sacrificed on day three. Quantification of Ki67+ EdU+ cells as a percenatge of total cells or -cells.(XLSX) pone.0159276.s005.xlsx (48K) GUID:?1CC24B83-32B5-47D7-82C5-0C6ED5295510 S6 Table: Individual data for islet cell, -cell, and non- islet cell counts & proliferation using Nkx6.1 as a marker for -cells. Measurements were recorded from 10-week-old CD1 male mice injected with maltose binding protein (MBP; control) or MBP-human betatrophin (hBT) fusion protein for two days and sacrificed on day three. Islet cells were identified by dilating the insulin area by one cells diameter and then filling all holes within the region of the object. -cells were identified by Nkx6.1+ cells co-localized with DAPI surrounded by insulin. Non- islet cells were 10-DEBC HCl calculated by subtracting the -cell counts from the total islet cell counts.(XLSX) pone.0159276.s006.xlsx (52K) GUID:?D7123D79-7FAC-4DE3-AE29-7311EA2807E0 S7 Table: Individual data for islet cell Ki67 proliferation based on stained slides described in Fig 3. Measurements were recorded from 10-week-old CD1 male mice injected with maltose binding protein (MBP; control) or MBP-human betatrophin (hBT) fusion protein for two days and sacrificed on day three. Islet 10-DEBC HCl cells were identified by dilating the insulin 10-DEBC HCl area by one cells diameter and then filling all holes within the region of the object. Quantification of total Ki67 proliferation (% of total DAPI+ islet cells).(XLSX) pone.0159276.s007.xlsx (50K) GUID:?9D452771-C894-4348-836D-80CA3E9C61F5 S8 Table: Individual data for exocrine cell proliferation for ANGPTL8 treatment studies. Measurements were recorded from 10-week-old CD1 male mice injected with maltose binding protein (MBP; control) or MBP-human betatrophin (hBT) fusion protein for two days and sacrificed on day three. Quantification of pancreatic proliferation by Ki67 or EdU (% of total exocrine cells). Exocrine cells were calculated by subtracting total DAPI+ islet cells from all DAPI cells.(XLSX) pone.0159276.s008.xlsx (50K) GUID:?99E0D01A-8179-4847-BE55-C0B05FD8BA10 Data Availability StatementAll relevant data are within the paper and its Supporting Information files. Abstract The -cell mitogenic effects of ANGPTL8 have been subjected to substantial debate. The original findings suggested that ANGPTL8 overexpression in mice induced a 17-fold increase in -cell proliferation. Subsequent studies in mice contested this claim, but a more recent report in rats supported the original observations. These conflicting Rabbit Polyclonal to STAT5B results may be explained by adjustable ANGPTL8 expression and various ways of -cell quantification. To resolve the controversy, three independent labs collaborated on a blinded study to test the effects of ANGPTL8 upon -cell proliferation. Recombinant human betatrophin (hBT) fused to maltose binding protein (MBP) was delivered to mice by intravenous injection. The results demonstrate that ANGPTL8 does not stimulate significant -cell proliferation. Each lab employed different methods for -cell identification, resulting in variable quantification of -cell proliferation and suggests a need for standardizing practices for -cell quantification. We also observed a new action of ANGPTL8 in stimulating CD45+ hematopoietic-derived cell proliferation which may explain, in part, published discrepancies..