Follow-up of the rest of the SCV-inoculated pets (n = 2 per group) revealed that that they had all seroconverted5 by 28 times p.i. hand civets ( em Paguma larvata /em ) and raccoon canines ( em Nyctereutes procyonoides /em ) could be infected using a pathogen that is nearly the same as SCV (ref. 3). Local felines surviving in the Amoy Backyards apartment obstruct in Hong Kong, where a lot more than 100 citizens contracted SARS this past year, had been discovered to become infected with SCV also. To check the susceptibility of local ferrets and felines to SCV infections, we inoculated them intratracheally with 106 TOK-001 (Galeterone) median tissue-culture infectious dosage units (TCID50), which we obtained from patient 5688 (who died from SARS) and then TOK-001 (Galeterone) passaged four times on Vero 118 cells4,5 em in vitro /em . We then took nasal, pharyngeal and rectal swabs from the animals on different days post-infection (p.i.). Four animals from each group were killed at 4 days p.i. and were necropsied according to a standard protocol4,5. No clinical signs were seen in SCV-inoculated cats, whereas three out of six ferrets became lethargic from days 2C4 p.i. and one of these ferrets died 4 days p.i. All cats (Fig. 1a) and ferrets (Fig. 1b) shed SCV from the pharynx, starting at 2 days p.i. and continuing until days 10 and 14, respectively, as demonstrated by polymerase chain reaction with reverse transcription (RT-PCR)5. The virus was isolated4,5 from all pharyngeal swabs taken on days 2C8 p.i. and from nasal swabs taken from two cats on days 4 and 6 p.i. SCV was not detected in nasal swabs from ferrets or in rectal swabs from cats or ferrets. Open in a separate window Figure 1 Daily excretion of Odz3 SARS coronavirus (SCV) in ferrets and domestic cats after inoculation with the virus or exposure to infected animals.a, b, SCV titres per ml from cats (a) and ferrets (b) (n = 6 of each) that had been inoculated with SCV through the respiratory route. Four animals from each group were killed on the fourth day after infection, and two TOK-001 (Galeterone) were kept until day 28. c, d, SCV titres from non-inoculated cats (c) and ferrets (d) (n = 2 of each) that had been housed with inoculated cats and ferrets, respectively. SCV excretion was quantified in pharyngeal swabs by using reverse transcription with the polymerase chain reaction, and was compared to a titrated SCV standard. ND, not determined. Infection of the respiratory tract was evident in all animals tested: SCV was isolated from the trachea and lungs (see supplementary information). Quantification of the viral titres in lung homogenates revealed relatively low SCV titres (geometric mean s.d.) in the lungs of SCV-inoculated cats (1 103 0.51 TCID50 ml?1) compared with those in ferrets (1 106 0.70 TCID50 ml?1). Histologically, SCV infection was associated with pulmonary lesions similar to those seen in SCV-infected macaques4,5, except that they were milder, particularly in SCV-infected cats, and did not feature syncytia. In the gastrointestinal and urinary tracts, SCV was detected by RT-PCR (see supplementary information). Follow-up of the remaining SCV-inoculated animals (n = 2 per group) revealed that they had all seroconverted5 by 28 days p.i. (neutralizing antibody titres of 40C320). Two attempts to infect suckling mice through intracerebral inoculation failed. Non-inoculated cats (Fig. 1c; n = 2) and ferrets (Fig. 1d; n = 2) that were housed with the inoculated cats and ferrets, respectively, became infected with SCV: viral titres gradually increased from 2 days p.i. onwards, peaking at days 6C8 p.i. Neither of the cats showed clinical signs of infection, but both had seroconverted by day 28 (they had virus-neutralizing antibody titres of 40 and 160, respectively). Both ferrets were lethargic and developed conjunctivitis; they died on days 16 and 21 p.i. We established by pathological examination that the main lesions in both animals were marked hepatic lipidosis and emaciation. There was no evidence that either of these animals died from SCV-associated pneumonia, although SCV was isolated from post-mortem lung specimens of one animal. Our results show that ferrets and domestic cats are susceptible to experimental infection by SCV, and.