During acute infection, the virus is secreted in urine and saliva for months. rates were be higher as compared with those reported in the literature. are very high and Mouse monoclonal to S100A10/P11 they may cause congenital malformations in the fetus by crossing the placental barrier(1). Miscarriage and stillbirth are the most serious consequences of congenital toxoplasmosis. If death does not occur, microcephaly, hydrocephaly, cerebral calcifications, convulsions and psychomotor retardation may develop in the fetus. The disease is milder in fetuses that are infected during later prenatal development. Maternal Rubella leads to massive defects including cardiac and ocular anomalies, deafness, and microcephaly in the fetus in the first trimester(2). Half of the primary infections encountered during pregnancy affects the fetus. Jaundice, hepatosplenomegaly, petechial rashes, hemolytic anemia, microcephaly, chorioretinitis, and cerebral calcifications may be seen in infants with fulminant cytomegalic inclusion disease. In Turkey, the rate of seropositivity of Toxoplasmosis IgM and IgG is reported to be 4-11% and 47-54%, respectively. In addition to that, and Rubella seropositivity rates are reported to be 55-91% and 65-90%, respectively(1,3). The present study aimed to determine the seroprevalence of Toxoplasma, Rubella, and infections among patients receiving prenatal care at Van Training and Research Hospital. MATERIALS AND METHODS Toxoplasma, Rubella and antibodies were analyzed in the serum samples of pregnant women receiving prenatal care in the Department of Gynecology and Obstetrics of Van Training and Research Hospital between June 2012 and July 2013, and the positive serum samples were retrospectively investigated. For this purpose, presence of anti-Toxoplasma IgM, anti-Toxoplasma IgG, anti-Rubella IgM, anti-Rubella IgG, anti-IgM, anti-IgG, anti-Toxoplasma IgG avidity and anti-IgG avidity were investigated. Only the initial results of each patient were taken into account and repeated recordings were excluded. Blood samples obtained for Xyloccensin K Xyloccensin K anti-Toxoplasma, anti-Rubella, and Xyloccensin K anti-antibody screening were centrifuged at 10 000 rpm for 15 min and then analyzed using enzyme-linked immunosorbent assay (ELISA) within 2 hours using Cobas 4000 e411 (Roche, Germany) and Architect i2000SR (Abbott Diagnostics, Germany) analyzers. Toxoplasma IgM values higher than 0.6 ratio and Toxoplasma IgG higher than 3 IU/ml were taken as positive. Rubella IgM values with a ratio above 1.6 and Rubella IgG values above 10 IU/ml were considered as positive. IgM values with a ratio greater than 1 and IgG values greater than 6 AU/mL were regarded as positive. Z test was used for the comparison of ratios for categorical variables. The level of statistical significance was considered to be 5% and MINITAB version 14 statistical package was used for analyses. The study was approved by the Yznc Y?l University Faculty of Medicine, Human Ethics Committee (30.01.2014/08). RESULTS Over the course of the study period, the results of a total of 9809 patients were analyzed in terms of anti-Toxoplasma, anti-Rubella, and anti-antibodies. The rates of anti-Toxoplasma, anti-Rubella, and anti-IgM antibody positivity were 1.1%, 0.5%, and 2.6%, respectively, whereas IgG antibody positivity was 37.6%, 86.5%, and 100%, respectively. Avidity test was performed in the serum samples of 54 patients with positive anti-Toxoplasma IgG antibody results, and 35 (64.8%) of these patients had a high avidity test result. The differences between Toxoplasma, Rubella, and IgM seropositivity rates were found to be statistically significant. Similarly, the differences between Toxoplasma, Rubella, and IgG, the differences between anti-Toxoplasma IgG avidity, and anti-IgG avidity seropositivity rates were determined to be statistically significant (p 0.01). Anti-Toxoplasma IgG avidity was low in 15 patients but was within normal ranges in.