The abdominal wall was open by a 5-cm midline incision, the left colon 8 cm above of anal margin was sectioned, and the cranial segment was exteriorized as a proximal colostomy. to intervention with SCF in greater concentration and for a longer period of intervention. There was an increase in tissue content of claudin-3 and occludin, related to SCF concentration. The tissue content of both proteins was not related to the intervention time. CONCLUSION: Enemas with SCF reduced the inflammation and increased the tissue content of claudin-3 and occludin in colonic mucosa without fecal stream. strong class=”kwd-title” HEADINGS: Colitis, Claudin-3, Occludin, Image processing, Computer-assisted, Sucralfate RESUMO – RACIONAL: O estresse oxidativo um dos principais mecanismos associados ruptura dos mecanismos de defesa que formam a barreira epitelial clica e reduz o contedo tecidual das protenas claudina-3 e ocludina principais constituintes das jun??es de oclus?o intercelulares. O sucralfato, possui atividade antioxidante e tem sido usado para tratar diferentes formas de colite. OBJETIVO: Mensurar o contedo tecidual de claudina-3 e ocludina da mucosa do clon sem transito fecal, submetido interven??o com sucralfato. MTODO: Trinta e seis ratos foram submetidos colostomia do clon esquerdo e fstula mucosa distal. Os animais foram divididos em dois grupos de acordo com a eutansia ser realizada duas ou quatro semanas aps a interven??o. Cada grupo foi dividido em trs subgrupos de acordo com o tipo de interven??o realizada diariamente: solu??o salina isolada; sucralfato a 1 g/kg/dia ou sucralfato a 2g/kg/dia. A colite foi diagnosticada por anlise histolgica adotando escala de valida??o prvia. A express?o tecidual de ambas as protenas foi identificada por imunoistoqumica. O contedo das protenas foi quantificado por anlise de imagem assistida por computador. RESULTADOS: O escore inflamatrio foi maior nos segmentos clicos sem transito fecal e os enemas com sucralfato reduziram o escore inflamatrio nesses segmentos, principalmente nos animais submetidos interven??o com sucralfato em maior concentra??o e por perodo mais longo de interven??o. Houve aumento no contedo tecidual das protenas claudina-3 e ocludina, relacionado com a concentra??o de sucralfato. O contedo HLC3 tecidual de ambas as protenas n?o se modificou com a dura??o da interven??o. CONCLUS?O: Enemas com sucralfato reduzem a inflama??o e aumentam o contedo tecidual de claudina-3 e ocludina na mucosa clica sem transito intestinal. strong class=”kwd-title” DESCRITORES: Colite, Claudina-3, Ocludina, Processamento de imagem PFK-158 assistida por computador, Sucralfato INTRODUCTION The colonic epithelium is the most important defensive barrier of the human body 1 . It consists of only a single layer of specialized cells and forms a PFK-158 highly dynamic and selective barrier that controls the absorption of fluid and solutes by restricting pathogen access to underlying tissues 7 , 31 . The cells of the colonic epithelium must sense and respond appropriately to the constant immunological challenge of the colonic luminal contents and, at the same time, need to allow absorption of water, nutrients, and molecules important for maintaining the cellular energy metabolism 1 . This efficient barrier function is achieved by a series of intercellular junctions that PFK-158 include apical tight junctions (TJs) and PFK-158 subjacent adherents junction, desmosomes, and gap junctions, which mediate intercellular adhesion and the communication between adjacent epithelial cells 14 . The mucus layer covers the colonic epithelium, the cytoplasmatic membrane of the cells that forms the colic glands, and basal membrane; immunoglobulins, cytokines, and leukocytes form the immune barrier against pathogens and participate in this mechanism of defense 14 , 22 . The TJs are the most apical component of the intercellular junctions systems and provide an efficient form of cell-cell adhesion in colonic epithelium 34 . They connect adjacent cells together to determine controlled paracellular permeability through the lateral intercellular space 34 . Increasing importance is being attributed to TJs in the mechanisms of cell proliferation, production of mucus, identification of antigens and pathogenic bacteria, and production of antimicrobial peptides to ensure effective immune cell differentiation 27 . TJs are composed of multiple proteins such as claudins family, occludin, tricellulin, and junctional adhesion molecule 15 . Mucosal inflammation as observed in inflammatory bowel disease compromises the epithelial barrier, resulting in the exposure of lamina propria tissue compartments to luminal antigens and microbes, thus contributing to the inflammatory response and epithelial-barrier defects 7 , 8 , 12 . An experimental study showed that in diversion colitis (DC), an inflammatory disease occurs in colonic.